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A5463
Sigma
Anti-Acetylated Proteins antibody produced in rabbit
~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| MDL number: | MFCD03454689 |
Description
| Immunogen | synthetic acetylated peptide corresponding to the N-terminus of human histone H4 (amino acids 1-20, with N-ε-acetylated lysines at positions 5,8,12 and 16) conjugated to KLH. |
| Specificity | Reacts with several acetylated proteins (e.g., acetylated histone H4 [12 kDa], acetylated H1 and H2b and acetylated BSA). Does not recognize acetylated histone H3. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| species reactivity | human, bovine, chicken |
| concentration | ~0.5 mg/mL |
| application(s) | dot blot: 1:1,000 using Acetylated Histones H4, H1, H2b and acetylated BSA |
| immunoblotting: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate | |
| indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells | |
| microarray: suitable | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
